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Becton Dickinson
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TSI Incorporated
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Bio-Rad
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LeCroy Corporation
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Eppendorf AG
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DePuy Synthes
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EdgeTech Instruments Inc
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Image Search Results
Journal: bioRxiv
Article Title: Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis
doi: 10.1101/471979
Figure Lengend Snippet: Quantification of band densitometry of (A) p-IGF1R Y 1135 , (B) p-ERK1/2 T 202 /Y 204 , (C) p-Akt S 473 , (D) p-p70S6K T 389 , (E) p-p70S6K T 421 /S 424 , (F) p-rpS6 S 235 /S 236 , normalized to total protein, following rotator cuff tear. (G) Representative phospho and total protein Western blots, with a Coomassie stained membrane as a loading control. Data are presented mean+SD, N=6 per group. Post-hoc sorting (P<0.05): a, different from 0d; b, different from 10d; c, different from 30d.
Article Snippet: Membranes were rinsed and incubated overnight in primary antibodies at a concentration of 1:1000, against either glutathione (Virogen, Watertown, MA, USA, 101A) succinate dehydrogenase-A (SDHA, Cell Signaling, Danvers, MA, USA, 11998), cytochrome c oxidase subunit 4 (COX4, Cell Signaling 4850), phospho-insulin-like growth factor-1 receptor (p-IGF1R) Y 1135 (Cell Signaling 3918), IGF1R (Cell Signaling 9750), phospho-ERK1/2 T 202 /Y 204 (Cell Signaling 4370), ERK1/2 (Cell Signaling 4695), phospho-Akt S 473 (Cell Signaling 4060), Akt (Cell Signaling 4691), phospho-p70S6 Kinase (p-p70S6K) T 389 (Cell Signaling 9234), phospho-p70 S6K T 421
Techniques: Western Blot, Staining